BME103:T930 Group 9: Difference between revisions
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<center>[[Image: BME103_Group9_Taq Polymerase.gif |500px|thumb|Summary: This is an image of how Taq polymerases amplify the DNA. [2]]]</center> Source: [http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif]<br> | <center>[[Image: BME103_Group9_Taq Polymerase.gif |500px|thumb|Summary: This is an image of how Taq polymerases amplify the DNA. [2]]]</center> Source: [http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif]<br> | ||
For more information on the PCR DNA replication process, please visit this website: http://learn.genetics.utah.edu/content/labs/pcr/ . | <br>For more information on the PCR DNA replication process, please visit this website: http://learn.genetics.utah.edu/content/labs/pcr/. | ||
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Revision as of 10:26, 8 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAMLAB 1 WRITE-UP(Please finish by 11/7/2012) Initial Machine TestingThe Original Design
When we unplugged part 3, the LCD, from part 6, the Open PCR circuit Board, the LCD on the machine turned off and no information appeared on the LCD screen.
Test Run On October 25 2012, we conducted our first test on our open PCR machine. We tested the machine to test the operation functionality. The initial test demonstrated the machine heat sink accuratly controlled and displayed the preprogrammed temperaute determined by the software on the computer. The overall successfullness of the machine was good, however it came with one difficulity, fluctuation of time to complete the preporgrammed cycles. (Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction (Add your work from Week 3, Part 1 here)
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology In Week 3 of our PCR experiment, we studied the cancer marker in the PCR experiment was correlated to the breast and colorectal cancer. Based on conditional probabilities, we found that the frequency of this cancer found in Finland was 7.8%. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.
Results(Your group will add the results of your Fluorimeter measurements from Week 4 here)
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