BME103:T930 Group 9 l2: Difference between revisions
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'''DNA Measurement Protocol'''<br> | |||
Fluorimeter Assembly <br> | |||
#Place a glass slide on the device. <br> | |||
'''DNA Measurement Protocol''' | #Using a pipette, add two drops of water to the slide. <br> | ||
#Turn on the blue LED light, and adjust the slide so that the light shines directly through the center of the water drop. <br> | |||
#Adjust the camera settings on a smartphone as follows: <br> | |||
#*Turn off the flash <br> | |||
#*Set exposure to the highest setting <br> | |||
#*Set saturation to the highest setting <br> | |||
#*Set contrast to the lowest setting <br> | |||
#Place the smartphone on the phone holder and position it in front the of fluorimeter device. <br> | |||
#Cover the entire setup with a black box in order to create as dark of an environment as possible. <br> | |||
#Take a picture with the smartphone. For best results, set the camera timer on the smartphone in order to be able to take a picture with the box completely closed. <br> | |||
==Research and Development== | ==Research and Development== |
Revision as of 17:23, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
The first of the two features is a better performing LCD screen with attached four analog buttons on the bottom of screen that allow for the programmer to program the machine and eliminate the need of a computer to program. The four analog buttons will include: Increase, Decrease, Temp/Time, Start/Stop/Restart. These buttons will allow the programmer to prepare a PCR experiment sequence without the use of external equipment, such as a computer equipped with PCR software. These buttons will be placed directly below the LCD screen, so that they can be easily accessible and usable. The second of the two features includes a new internal PCR Board, which is configured with the new LCD screen. Since the programmer will now be able to change the program on the machine itself, the internal PCR Board will have to be modified to allow the program to be stored and set in the PCR Board. The changes that need to be taken with this equipment are to wire the buttons from the LCD screen to the PCR Board, so that the inputs by the user can be recognized by the system and the PCR board will also need a new program implemented into the system that can understand the user inputs as well. With these small design changes, we will eliminate the reliance on other technologies to operate the machine and increase its overall mobility. Instructions
ProtocolsMaterials
Supplied by user
PCR Protocol
DNA Measurement Protocol
Research and DevelopmentBackground on Disease Markers
The marker that is being used is rs709932[2].This SNP is associated with Emphysema due to AAT deficiency. The sequence associated with Emphysema due to AAT deficiency is C(A)T , while a normal sequence is C(G)T, which is located on the 14 chromosome. The gene alteration leads to a mutated human protein. It goes from R[Arg] to H[His].
Emphysema due to AAT deficiency
The marker that is being used is rs137852466 [4]. This SNP is associated with Hemophilia A. The sequence associated with Hemophilia A is C(G)C , while a normal sequence is C(A)C, which is located on the X chromosome.The gene alteration leads to a mutated human protein. It goes from R[Arg] to H[His].
Primer Design
Illustration
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