BME103:TEMPwu1

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==Results==
==Results==
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<!--- Place two small images here. One showing Water in Image J and one showing Calf Thymus DNA --->
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{| class="wikitable" style="width:500px; cellspacing:2px; cellpadding=2px;"
{| class="wikitable" style="width:500px; cellspacing:2px; cellpadding=2px;"
|- bgcolor="grey"
|- bgcolor="grey"
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|  '''Sample''' || '''INTDEN''' || '''DNA μg/mL''' || '''Conclusion'''
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|  '''Sample''' || '''Integrated Density''' || '''DNA μg/mL''' || '''Conclusion'''
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|-
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| cell1 || cell 2 || cell 3 || cell 4
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| PCR: Negative Control || E6 || F6 || G6
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|-
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| cell 4 || cell 5 || cell 6 || cell 7
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| PCR: Positive Control || E7 || F7 || G7
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|-
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| PCR: Patient ID #####, rep 1 || E8 || F8 || G8
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|-
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| PCR: Patient ID #####, rep 2 || E9 || F9 || G9
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|}
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KEY
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* '''Sample''' = <!--- explain what "sample" means --->
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* '''Integrated Density''' = <!--- explain what "integrated density" means and how you did background subtraction to get this value --->
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* '''DNA μg/mL''' = <!--- how you calculated this --->
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* '''Conclusion''' = <!--- explain

Revision as of 04:39, 9 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
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Image:BME494_Asu_logo.png

Contents

OUR TEAM

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LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

(Add your work from Week 3, Part 1 here)


Flourimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

Sample Integrated Density DNA μg/mL Conclusion
PCR: Negative Control E6 F6 G6
PCR: Positive Control E7 F7 G7
PCR: Patient ID #####, rep 1 E8 F8 G8
PCR: Patient ID #####, rep 2 E9 F9 G9

KEY

  • Sample =
  • Integrated Density =
  • DNA μg/mL =
  • Conclusion =
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