BME103:TEMPwu1

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=LAB 1 WRITE-UP=
=LAB 1 WRITE-UP=
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(Please finish by 11/7/2012)
 
==Initial Machine Testing==
==Initial Machine Testing==
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-
(Write the date you first tested Open PCR and your experience(s) with the machine)<br>
 
'''The Original Design'''<br>
'''The Original Design'''<br>
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)<br>
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)<br>
 +
 +
 +
'''Experimenting With the Connections'''<br>
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 +
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)
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 +
When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
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 +
 +
'''Test Run'''
 +
 +
(Write the date you first tested Open PCR and your experience(s) with the machine)<br>
<br><br>
<br><br>
 +
==Protocols==
==Protocols==
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'''Flourimeter Measurements'''<br>
'''Flourimeter Measurements'''<br>
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(Add your work from Week 3, Part 1 here)<br>
+
(Add your work from Week 3, Part 2 here)<br>
 +
<br><br>
==Research and Development==
==Research and Development==
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'''Specific Cancer Marker Detection - The Underlying Technology'''<br>
 +
 +
(Add a write-up of the information discussed in Week 3's class)<br>
 +
 +
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
 +
 +
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<br><br>
==Results==
==Results==
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(Your group will add the results of your Fluorimeter measurements here after Week 4)
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<!--- Place two small Image J data images here. One showing the result of Water and the other showing the result of Calf Thymus DNA --->
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<!--- Enter the values from your group's Data Analyzer table below. E6, F6, etc. are the excel cells from which you should copy your data. --->
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{| {{table}}
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|- style="background:#f0f0f0;"
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| '''Sample''' || '''Integrated Density''' || '''DNA μg/mL''' || '''Conclusion'''
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|-
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| PCR: Negative Control || E6 || F6 || G6
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|-
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| PCR: Positive Control || E7 || F7 || G7
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|-
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| PCR: Patient 1 ID #####, rep 1 || E8 || F8 || G8
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|-
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| PCR: Patient 1 ID #####, rep 2 || E9 || F9 || G9
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|-
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| PCR: Patient 1 ID #####, rep 3 || E10 || F10 || G10
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|-
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| PCR: Patient 2 ID #####, rep 1 || E11 || F11 || G11
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|-
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| PCR: Patient 2 ID #####, rep 2 || E12 || F12 || G12
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|-
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| PCR: Patient 2 ID #####, rep 3 || E13 || F13 || G13
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|}
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 +
KEY
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* '''Sample''' = <!--- explain what "sample" means --->
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* '''Integrated Density''' = <!--- explain what "integrated density" means and how you did background subtraction to get this value --->
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* '''DNA μg/mL''' = <!--- explain how you calculated this --->
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* '''Conclusion''' = <!--- explain what "Positive" and "No signal" means, relative to the control samples --->

Revision as of 16:22, 9 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

(Add your work from Week 3, Part 1 here)


Flourimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

Sample Integrated Density DNA μg/mL Conclusion
PCR: Negative Control E6 F6 G6
PCR: Positive Control E7 F7 G7
PCR: Patient 1 ID #####, rep 1 E8 F8 G8
PCR: Patient 1 ID #####, rep 2 E9 F9 G9
PCR: Patient 1 ID #####, rep 3 E10 F10 G10
PCR: Patient 2 ID #####, rep 1 E11 F11 G11
PCR: Patient 2 ID #####, rep 2 E12 F12 G12
PCR: Patient 2 ID #####, rep 3 E13 F13 G13


KEY

  • Sample =
  • Integrated Density =
  • DNA μg/mL =
  • Conclusion =


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