BME103:TEMPwu1

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(Protocols)
(Protocols)
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'''Thermal Cycler Program'''<br>
'''Thermal Cycler Program'''<br>
 +
'''DNA Sample Set-up'''<br>
'''DNA Sample Set-up'''<br>
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| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
|}
|}
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'''DNA Sample Set-up Procedure'''
'''DNA Sample Set-up Procedure'''
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# Step 2
# Step 2
# Step 3...
# Step 3...
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'''PCR Reaction Mix'''
'''PCR Reaction Mix'''
* What is in the PCR reaction mix?
* What is in the PCR reaction mix?
 +
'''DNA/ primer mix'''
'''DNA/ primer mix'''
* What is in the DNA/ primer mix?
* What is in the DNA/ primer mix?
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Revision as of 14:01, 19 March 2013

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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Contents

OUR TEAM

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LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Thermal Cycler Program


DNA Sample Set-up

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


PCR Reaction Mix

  • What is in the PCR reaction mix?


DNA/ primer mix

  • What is in the DNA/ primer mix?





Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)





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