BME103:TEMPwu1: Difference between revisions
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'''The Original Design'''<br> | '''The Original Design'''<br> | ||
(Add image of the full OpenPCR machine here, from the Week | (Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)<br> | ||
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'''Polymerase Chain Reaction'''<br> | '''Polymerase Chain Reaction'''<br> | ||
(Add your work from Week 3, Part 1 here)<br> | |||
'''Flourimeter Measurements'''<br> | '''Flourimeter Measurements'''<br> | ||
(Add your work from Week 3, Part 2 here)<br> | |||
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<br><br> | <br><br> | ||
==Results== | |||
<!--- Place two small Image J data images here. One showing the result of Water and the other showing the result of Calf Thymus DNA ---> | |||
<!--- Enter the values from your group's Data Analyzer table below. E6, F6, etc. are the excel cells from which you should copy your data. ---> | |||
{| {{table}} | |||
|- style="background:#f0f0f0;" | |||
| '''Sample''' || '''Integrated Density''' || '''DNA μg/mL''' || '''Conclusion''' | |||
|- | |||
| PCR: Negative Control || E6 || F6 || G6 | |||
|- | |||
| PCR: Positive Control || E7 || F7 || G7 | |||
|- | |||
| PCR: Patient 1 ID #####, rep 1 || E8 || F8 || G8 | |||
|- | |||
| PCR: Patient 1 ID #####, rep 2 || E9 || F9 || G9 | |||
|- | |||
| PCR: Patient 1 ID #####, rep 3 || E10 || F10 || G10 | |||
|- | |||
| PCR: Patient 2 ID #####, rep 1 || E11 || F11 || G11 | |||
|- | |||
| PCR: Patient 2 ID #####, rep 2 || E12 || F12 || G12 | |||
|- | |||
| PCR: Patient 2 ID #####, rep 3 || E13 || F13 || G13 | |||
|} | |||
KEY | |||
* '''Sample''' = <!--- explain what "sample" means ---> | |||
* '''Integrated Density''' = <!--- explain what "integrated density" means and how you did background subtraction to get this value ---> | |||
* '''DNA μg/mL''' = <!--- explain how you calculated this ---> | |||
* '''Conclusion''' = <!--- explain what "Positive" and "No signal" means, relative to the control samples ---> | |||
Latest revision as of 20:04, 21 March 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction (Add your work from Week 3, Part 1 here)
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
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