BME103:TEMPwu2: Difference between revisions
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==Protocols== | ==Protocols== | ||
' | <!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method | ||
Things to consider: | |||
How should the PCR machine be set up? Does it need to be plugged in? | |||
How many samples will fit into a single 2-hour run? | |||
How many replicates should be created per patient? | |||
What should the final volume of the reaction be? | |||
Will signal reading be integrated into the PCR machine or remain separate? | |||
If it is separate, you will need to include instructions on how to use the fluorimeter. | |||
How should the user calculate the about of signal amplified? | |||
etc. | |||
---> | |||
''' | '''Example List''' | ||
# Step 1 | |||
# Step 2 | |||
# Step 3 | |||
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<!--- Explain how PCR will be used to amplify signal from your target of choice and why the test will produce no signal if the target is absent ---> | <!--- Explain how PCR will be used to amplify signal from your target of choice and why the test will produce no signal if the target is absent ---> | ||
<!--- Explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers ---> | |||
Revision as of 02:06, 9 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAMLAB 2 WRITE-UP(Please finish by 11/28/2012)
Thermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
ProtocolsExample List
Research and Development |