BME103:W930 Group1

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Kevin Chu Experimental Protocol Planner Name: Michael Dennison Experimental Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 1 WRITE-UP (Please finish by 11/7/2012) Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged part (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction Polymerase Chain Reaction Polymerase Chain Reaction Reagent Volume Template DNA (20 ng) 0.1μL 10μM forward primer 0.5μL 10μM reverse primer 0.5μL GoTaq master mix 25.0μL dH2O 23.9μL Total Volume 50.0μL Positive Control Cancer DNA template Negative Control DNA Template Patient 1 Replicate 1 ID: 92336 Gender: Male Age: 58 Patient 1 Replicate 2 ID: 92336 Gender: Male Age: 58 Patient 1 Replicate 3 ID: 92336 Gender: Male Age: 58 Patient 2 Replicate 1 ID: 44606 Gender: Male Age: 47 Patient 2 Replicate 2 ID: 44606 Gender: Male Age: 47 Patient 2 Replicate 3 ID: 44606 Gender: Male Age: 47 Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)