BME103:W930 Group1

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Kevin Chu Experimental Protocol Planner Name: Michael Dennison Experimental Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 1 WRITE-UP (Please finish by 11/7/2012) Initial Machine Testing The Original Design ) Original design of the Open PCR machine showing inner mechanisms. While it is portable and easy to use, the design is fragile and has a high failure rate, along with several other design flaws. Experimenting With the Connections When we unplugged part LCD screen from the circuit board, the machine's screen stopped displaying. When we unplugged the white wire that connects the circuit board to the heated lid, the machine stopped controlling the temperature. Test Run During our first test run on October 24, 2012, the machine's fan would not work and therefore we could not complete the DNA replication. Protocols Polymerase Chain Reaction Polymerase Chain Reaction Polymerase Chain Reaction Reagent Volume Template DNA (20 ng) 0.1μL 10μM forward primer 0.5μL 10μM reverse primer 0.5μL GoTaq master mix 25.0μL dH2O 23.9μL Total Volume 50.0μL Positive Control Cancer DNA template Negative Control DNA Template Patient 1 Replicate 1 ID: 92336 Gender: Male Age: 58 Patient 1 Replicate 2 ID: 92336 Gender: Male Age: 58 Patient 1 Replicate 3 ID: 92336 Gender: Male Age: 58 Patient 2 Replicate 1 ID: 44606 Gender: Male Age: 47 Patient 2 Replicate 2 ID: 44606 Gender: Male Age: 47 Patient 2 Replicate 3 ID: 44606 Gender: Male Age: 47 Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)