BME103:W930 Group2: Difference between revisions
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==Results== | ==Results== | ||
Buffer 3 ----------------1440716------------------------------0.5 <br> | Buffer 3 ----------------1440716------------------------------0.5 <br> | ||
Buffer 4-----------------2814775------------------------------ 0.8 <br> | Buffer 4-----------------2814775------------------------------ 0.8 <br> | ||
<!--- Place two small images here. One showing Water in Image J and one showing Calf Thymus DNA ---> | <!--- Place two small images here. One showing Water in Image J and one showing Calf Thymus DNA ---> | ||
<!--- Enter the values from your group's Data Analyzer table below. E6, F6, etc. are the excel cells from which you should copy your data. ---> | <!--- Enter the values from your group's Data Analyzer table below. E6, F6, etc. are the excel cells from which you should copy your data. ---> | ||
{| {{table}} | {| {{table}} | ||
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| '''Sample''' || '''Integrated Density''' || '''DNA μg/mL''' || '''Conclusion''' | | '''Sample''' || '''Integrated Density''' || '''DNA μg/mL''' || '''Conclusion''' | ||
|- | |- | ||
| PCR: Negative Control || 218527 || 0 || | | PCR: Negative Control || 218527 || 0 || Water | ||
|- | |- | ||
| PCR: Positive Control || 6369082 || 2 || | | PCR: Positive Control || 6369082 || 2 || Calf Thymus | ||
|- | |- | ||
| PCR: Patient 1 ID 43825, rep 1 || 1193961 || 0 || G8 | | PCR: Patient 1 ID 43825, rep 1 || 1193961 || 0 || G8 | ||
|- | |- | ||
| PCR: Patient 1 ID 43825, rep 2 || | | PCR: Patient 1 ID 43825, rep 2 || 5237029 || 1.1 || G9 | ||
|- | |- | ||
| PCR: Patient 1 ID 43825, rep 3 || | | PCR: Patient 1 ID 43825, rep 3 || 5437872 || 1.2 || G10 | ||
|- | |- | ||
| PCR: Patient 2 ID 12079, rep 1 || | | PCR: Patient 2 ID 12079, rep 1 || 10726518 || 4 || G11 | ||
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| PCR: Patient 2 ID 12079, rep 2 || | | PCR: Patient 2 ID 12079, rep 2 || 2711977 || 0.7 || G12 | ||
|- | |- | ||
| PCR: Patient 2 ID 12079, rep 3 || | | PCR: Patient 2 ID 12079, rep 3 || 7637000 || 2.2 || G13 | ||
|} | |} | ||
Revision as of 10:59, 14 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine Testing
When we unplugged part mounting plate from circuit board, the machine had no visible screen and we could not see the the information on the LCD screen. When we unplugged the white wire that connects the circuit board to the sample holder, the machine's temperature was unable to be monitored and could not be regulated
Our group did our test run on October 24, 2012. During the test run, we ran into no problems with the machine. We were done and found our results in a matter of 1 to 2 hours. The Open PCR machine was connected to one computers to control each temperature of each cycle of PCR during the experimental.
ProtocolsPolymerase Chain Reaction How PCR Works: Step 1: Denaturation by Heat
1. A PCR Machine is created to amplify a patient’s DNA sample. In order to use a PCR machine, the steps include to first gather the DNA samples, 50 μL each.
400μM dATP, 400μM dGTP, 400μM dCTP, 400μM dTTP and 3mM MgCl2, reaction buffer (pH 8.5), nuclease-free water
Fluorimeter assembly procedure: Turn on the excitation light on the fluorimeter. Put a smart phone in the cradle and set it up to take pictures of the slide. Place two drops of water in the middle of the first two rows of the slide using a pipette. Align the drop by moving the slide so the drop is in the middle of the black fiber optic fitting. Cover the fluorimeter with the light box while still being able to use the smart phone to take pictures.
How to open images in Image J: Save the pictures to the phone. Download the pictures onto a computer that has Image J. Open them with Image J by going to add image. Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology Cancer Associated with the gene: Breast and Colorectal cancer with a susceptibility to LiFraumeni Syndrome Source: http://omim.org/entry/604373?search=CHEK2&highlight=chek2
Cancer Gene Sequence: Baye's Rule:
ResultsBuffer 3 ----------------1440716------------------------------0.5
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