BME103:W930 Group4 l2

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Contents

OUR TEAM

Name: StudentRole(s)
Name: Student
Role(s)
Name: Jake KrammerResearch and Development(s)
Name: Jake Krammer
Research and Development(s)
Name: Jan SimperResearch and Development
Name: Jan Simper
Research and Development
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
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Name: StudentRole(s)
Name: Student
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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers

Our group decided to look at two different diseases, metabolic syndrome and cardiovascular disease. Metabolic syndrome is a name for a group of risk factors that occur together and increase the risk for coronary artery disease, stroke, and type 2 diabetes. Cardiovascular disease refers to any disease that affects the cardiovascular system, principally cardiac disease, vascular diseases of the brain and kidney, and peripheral arterial disease. The associated mutation for metabolic syndrome was rs1800206, and for cardiovascular disease it was rs1801253.



Primer Design

Forward Primer:

Reverse Primer:



Illustration


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