BME103:W930 Group5 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Andrea Carpenter Role(s): Experimental Protocol Planner Name: Malik McLaurin Role(s): Open PCR Machine Engineer Name: Dana McElwain Role(s): Open PCR Machine Engineer Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials Supplied in Kit Supplies Amount Micro Test Tubes ? Transfer Pipettes ? PCR Master Mix 6 samples (598.8μL total) Positive Control Solution* 1 sample (100.0μL***) Negative Control Solution** 1 sample (100.0μL***) PCR Machine 1 Fluorimeter 1 (*)Positive control consists of calf thymus DNA (**)Negative control simply consists of a blank solution of water (***)Already mixed with PCR master mix Included in Fluorimeter Package: Supplies Amount Smart phone stand 1 LCD Box 1 Light box 1 Sybr green solution 500.0μL Components of PCR master mix: DNA Solution Component Amount Patient’s Template DNA* 0.2μL 10μM forward primer 1.0μL 10μM reverse primer 1.0μL Promega GoTaq master mix 50.0μL dH2O 47.8μL Total 100.0μL (*)Not actually included in kit, but must be added to the master mix by the user. Supplied by User Supplies Amount Smart Phone with Camera 1 Patient's Template DNA 6 samples (0.2μL each) External Computer 1 Image J Software 1 Open PCR Software 1 Gloves 1 pair Lab Coat 1 PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration