BME103:W930 Group5 l2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 58: | Line 58: | ||
---> | ---> | ||
'''Materials''' | <u>'''Materials'''</u><br> | ||
<br> | |||
<b>Supplied in the kit</b><br> | |||
<i>Components of PCR master mix:</i> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''DNA Solution Component''' | |||
| align="center" style="background:#f0f0f0;”|'''Amount''' | |||
|- | |||
| Patient’s Template DNA*||0.2μL | |||
|- | |||
| 10μM forward primer||1.0μL | |||
|- | |||
| 10μM reverse primer||1.0μL | |||
|- | |||
| GoTaq master mix||50.0μL | |||
|- | |||
| dH<sub>2</sub>O||47.8μL | |||
|- | |||
| Total||100.0μL | |||
|}<br> | |||
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here ---> | <!--- Place your two tables "Supplied in the kit" and "Supplied by User" here ---> | ||
Line 69: | Line 88: | ||
'''DNA Measurement Protocol''' | '''DNA Measurement Protocol''' | ||
==Research and Development== | ==Research and Development== |
Revision as of 22:52, 25 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
|