BME103:W930 Group5 l2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (→Protocols) |
|||
Line 78: | Line 78: | ||
|- | |- | ||
| Fluorimeter||1 | | Fluorimeter||1 | ||
|} | |} | ||
(*)Positive control consists of calf thymus DNA<br> | (*)Positive control consists of calf thymus DNA<br> | ||
(**)Negative control simply consists of a blank solution of water<br> | (**)Negative control simply consists of a blank solution of water<br> | ||
(***)Already mixed with PCR master mix | (***)Already mixed with PCR master mix<br><br> | ||
<i>Included in Fluorimeter Package</i><br> | <i>Included in Fluorimeter Package:</i><br> | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Supplies''' | | align="center" style="background:#f0f0f0;"|'''Supplies''' | ||
Line 114: | Line 113: | ||
|- | |- | ||
| Total||100.0μL | | Total||100.0μL | ||
|} | |} | ||
*Not actually included in kit, but must be added to the master mix by the user. | (*)Not actually included in kit, but must be added to the master mix by the user.<br><br> | ||
<b>Supplied by User</b><br> | <b>Supplied by User</b><br> |
Revision as of 23:22, 25 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
(*)Positive control consists of calf thymus DNA Included in Fluorimeter Package:
Components of PCR master mix:
(*)Not actually included in kit, but must be added to the master mix by the user. Supplied by User
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
|