BME103:W930 Group6 l2: Difference between revisions
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| [[Image:ME.jpg|100px|thumb|Name: Saraih<br>Machine Engineer(s)]] | | [[Image:ME.jpg|100px|thumb|Name: Saraih<br>Machine Engineer(s)]] | ||
| [[Image:Photo on 11-13-12 at 9.49 PM.jpg|100px|thumb|Name: Joshua<br>Research]] | | [[Image:Photo on 11-13-12 at 9.49 PM.jpg|100px|thumb|Name: Joshua<br>Research]] | ||
| [[Image: | | [[Image:GayaPieBeach.jpg|100px|thumb|Name: Kim<br>Reasearch and Development(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: Student<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: Student<br>Role(s)]] |
Revision as of 08:13, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features (Content by Sairah F) Instructions (Content by Sairah F)
ProtocolsMaterials Supplied in the Kit ---- Amount Supplied by User-----Amount
Fluorometer Use Research and DevelopmentBackground on Disease Markers This experiment heavily employs the use of PCR, or polymerase chain reaction. This process is used to amplify a segment of DNA for in-depth analysis. The process starts by adding a mix of DNA primers and DNA florescent tags that bind to a specific segment of DNA. After these primers bind, DNA polymerase binds to the primer-DNA strand bond and incorporates the florescent tags into the molecule. The polymerase adds base pairs to the base DNA strand and creates an antisense copy of the original strand. Since this process happens for both strands of the DNA, each process essentially duplicates the DNA strand. In order for this reaction to occur, the solution needs to be heated up to allow the double stranded DNA to melt into single strands. Then the solution needs to cool down and let the DNA anneal. Each one of these heating and cooling steps makes up the process of thermal cycling. Each cycle the DNA is replicated once. The sample is run through anywhere between 30 and 50 cycles in order to amplify the DNA and the targeted signal enough to be picked up by an imaging machine. Since the primers only bind to specific segments of DNA, the sample will only light up when the segment of interest is duplicated. In this lab, the SYBER green dye colored the DNA in solution, only fluorescing when bonded to double-stranded DNA. As a result, PCR can be used to identify the presence or absence of a particular DNA sequence. (content by William L.) "SNP" rs1042522 is a variant in the TP53 gene. The protein p53 is coded by the TP53 gene and acts as a tumor suppressor by regulating uncontrolled cell division and growth. When the DNA in a cell becomes damaged p53 plays a critical role in determining whether the DNA will be repaired or if the damaged cell will be destroyed. If the DNA can be repaired, p53 activates other genes to fix the damage. Otherwise,if the DNA cannot be repaired, this protein prevents the cell from dividing and signals it to undergo apoptosis. This process prevents cells with mutated or damaged DNA from dividing, which helps prevent the development of tumors. SNP rs1042522 is located on chromosome 17, position 7520197 Allele Change: CCC ⇒ CGC Primer Design In order to isolate this specific segment, two specific primers are required. The forward primer coding has the following sequence: 5'-ATGAAGCTCCCAGAATGC-3' The backward primer that attaches to the other strand of the DNA has the following sequence: 3' GCCGGTGTAGGAGCT 5' Both of these primers follow the standards for effective primers, having an annealing temperature of 61 degrees Celsius for the forward-generated strand and 59 degrees Celsius for the backward-generated strand. "'Link to NCBI webpage'" rs1042522
Bayesian Stats The relevance within Bayesian Stats is seen with the outcome of previous experiments to conclude a promising route when devising a new experiment using relatively the same equipment and testing methods. Withing the Bayesian statistics a percentage is given to guide the users on the accuracy of the experiment about to be attempted to give a good indication for what the results of the experiment will yield. Overall Bayesian statistics are especially useful when trying to get a statistical reading for the pros and cons of the certain tests throughout the experimentation period.
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