BME103:W930 Group6 l2

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(Protocols)
(Protocols)
Line 60: Line 60:
'''Materials'''
'''Materials'''
-
'''Supplied in the Kit''' ---- '''Amount'''
+
'''Supplied in the Kit''' ---- '''Amount'''<br>
-
10 μM Reverse Primer---7.5μL
+
10 μM Reverse Primer---7.5μL<br>
-
10 μM Forward Primer---7.5μL
+
10 μM Forward Primer---7.5μL<br>
-
GoTaq master mix---360μL
+
GoTaq master mix---360μL<br>
-
dH<sub>2</sub>O---360μL
+
dH<sub>2</sub>O---360μL<br>
-
'''Total Volume'''---750μL
+
'''Total Volume'''---750μL<br>

Revision as of 21:30, 27 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

Name: AntonioExperimental Protocol Planner
Name: Antonio
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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit ---- Amount
10 μM Reverse Primer---7.5μL
10 μM Forward Primer---7.5μL
GoTaq master mix---360μL
dH2O---360μL
Total Volume---750μL


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


Personal tools