BME103:W930 Group7

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(OUR TEAM)
(OUR TEAM)
Line 13: Line 13:
{| style="wikitable" width="700px"
{| style="wikitable" width="700px"
|- valign="top"
|- valign="top"
-
| [[Image:BME103student.jpg|100px|thumb|Name: Jake Turner<br>PCR Machine Engineer]]
+
| [[Image:BME103 Group7 Jake.jpg|100px|thumb|Name: Jake Turner<br>PCR Machine Engineer]]
-
| [[Image:BME103student.jpg|100px|thumb|Name: Tyler Allen<br>PCR Machine Engineer]]
+
| [[Image:BME103 Group7 Tyler.jpg|100px|thumb|Name: Tyler Allen<br>PCR Machine Engineer]]
-
| [[Image:BME103student.jpg|100px|thumb|Name: Khalil Pathan<br>Experimental Protocol Planner]]
+
| [[Image:BME103 Group7 Khalil.jpg|100px|thumb|Name: Khalil Pathan<br>Experimental Protocol Planner]]
-
| [[Image:BME103 Pahul.jpg|100px|thumb|Name: Pahul Singh<br>Experimental Protocol Planner]]
+
| [[Image:BME103 Group7 Pahul.jpg|100px|thumb|Name: Pahul Singh<br>Experimental Protocol Planner]]
| [[Image:FreaMehta.jpg|100px|thumb|Name: Frea Mehta<br>Research and Development Specialist]]
| [[Image:FreaMehta.jpg|100px|thumb|Name: Frea Mehta<br>Research and Development Specialist]]
| [[Image:BME103 Group7 Paul.jpg‎|100px|thumb|Name: Paul Song<br>Research and Development Specialist]]
| [[Image:BME103 Group7 Paul.jpg‎|100px|thumb|Name: Paul Song<br>Research and Development Specialist]]

Revision as of 22:10, 13 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: Jake TurnerPCR Machine Engineer
Name: Jake Turner
PCR Machine Engineer
Name: Tyler AllenPCR Machine Engineer
Name: Tyler Allen
PCR Machine Engineer
Name: Khalil PathanExperimental Protocol Planner
Name: Khalil Pathan
Experimental Protocol Planner
Name: Pahul SinghExperimental Protocol Planner
Name: Pahul Singh
Experimental Protocol Planner
Name: Frea MehtaResearch and Development Specialist
Name: Frea Mehta
Research and Development Specialist
Name: Paul SongResearch and Development Specialist
Name: Paul Song
Research and Development Specialist

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
Open PCR Machine

Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged part (part 3) from (part 6), the machine did not have power. The blue display screen did not turn on and appeared completely black.

When we unplugged the white wire that connects (part 6) to (part 2), the machine temperature on the display screen appeared incorrectly. Part 6 is responsible for recording the the internal temperature of the machine throughout the test.


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

(Add your work from Week 3, Part 1 here)


Flourimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

The NCBI database is used to isolate the sequence used and determine specific primers.

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

Sample Integrated Density DNA μg/mL Conclusion
PCR: Negative Control 267793 F6 G6
PCR: Positive Control 27409968 F7 G7
PCR: Patient 1 ID 91562, rep 1 3511064 F8 G8
PCR: Patient 1 ID 91562, rep 2 15099598 F9 G9
PCR: Patient 1 ID 91562, rep 3 8451848 F10 G10
PCR: Patient 2 ID 25235, rep 1 17311845 E12 G11
PCR: Patient 2 ID 25235, rep 2 9289657 E13 G12
PCR: Patient 2 ID 25235, rep 3 28825322 F13 G13


KEY

  • Sample =
  • Integrated Density =
  • DNA μg/mL =
  • Conclusion =
Personal tools