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PCR and the
Revision as of 21:14, 13 November 2012
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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LAB 1 WRITE-UP
Initial Machine Testing
Write a paragraph description for visitors who have no idea what this is)
When we unplugged part (part 3) from (part 6), the machine did not have power. The blue display screen did not turn on and appeared completely black.
When we unplugged the white wire that connects (part 6) to (part 2), the machine temperature on the display screen appeared incorrectly. Part 6 is responsible for recording the the internal temperature of the machine throughout the test.
While running our open PCR test, we experienced nothing but problems. We set the cycles to the appropriate temperatures and time intervals; the Initial cycle on 95°C for 30 seconds, the Denaturing cycle on 95°C for 30 seconds, the Annealing cycle on 55°C for 30 seconds, the Extending cycle on 72°C for 60 seconds, and the final hold at 20°C.
Polymerase Chain Reaction
(Add your work from Week 3, Part 1 here)
(Add your work from Week 3, Part 2 here)
Research and Development
Specific Cancer Marker Detection - The Underlying Technology
The NCBI database is used to isolate the sequence used and determine specific primers.
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)