BME103:W930 Group8: Difference between revisions

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(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)


{| class="wikitable" style="font-style:italic; font-size:120%; border:3px dashed red;"
|
|-
| Reagent ||    Volume
|-
| Template DNA (20 ng) ||||    0.2 μL
|-
| 10 μM Forward Primer ||||    1.0μL
|-
| 10 μM Reverse Primer ||||    1.0μL
|-
| GoTaq master mix ||||    50μL
|-
| dH<sub>2</sub>O ||||    47.8 μL
|-
| Total Volume ||||    100.0 μL
|}


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Revision as of 22:20, 1 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

Group 8 PCR Gurus

David Barclay
R&D Specialist
Alison Van Putten
R&D Specialist
Naomi Welch
Protocol Planner
Paige Stokes
Protocol Planner
Suzi Badgett
Machine Engineer
James Beauchamp
Machine Eng

LAB 1 WRITE-UP

(Please finish by 11/7/2012)

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged part (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

(Add your work from Week 3, Part 1 here)


Flourimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

()

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

(Your group will add the results of your Fluorimeter measurements from Week 4 here)