BME103:W930 Group8 l2: Difference between revisions

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m (BME103:W930 Group8 8 moved to BME103:W930 Group8 l2: To make BME103 Lab Write-up 2 page names consistent with the rest of the class.)
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'''Materials'''
'''Materials'''
{| class="wikitable"
|-
! Supplied in the Kit
! Amount
|-
| 10μM Forward Primer
| 25μL
|-
| 10μM Reverse Primer
| 2.5μL
|-
| GoTaq Master Mix
| 125μL
|-
| dH2O
| 119.5μL
|-
| Total Volume
| 250μL
|}
{| class="wikitable"
|-
! Suplied by User
! Amount
|-
| Sample of DNA by user (20ng)
| .5μL
|}


<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
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'''DNA Measurement Protocol'''
'''DNA Measurement Protocol'''


==Research and Development==
==Research and Development==

Revision as of 20:02, 26 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: Student
Role(s)
Name: Student
Role(s)
Name: Student
Role(s)
Name: Student
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Name: Student
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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
10μM Forward Primer 25μL
10μM Reverse Primer 2.5μL
GoTaq Master Mix 125μL
dH2O 119.5μL
Total Volume 250μL
Suplied by User Amount
Sample of DNA by user (20ng) .5μL



PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration