BME103:W930 Group8 l2: Difference between revisions

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Revision as of 20:03, 26 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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OUR TEAM

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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
10μM Forward Primer 25μL
10μM Reverse Primer 2.5μL
GoTaq Master Mix 125μL
H2O 119.5μL
Total Volume 250μL


Suplied by User Amount
Sample of DNA by user (20ng) .5μL



PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration