BME103:W930 Group8 l2: Difference between revisions

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'''Materials'''
'''Materials'''


{| class="wikitable"
{| class="wikitable" style="font-style:italic; font-size:120%; border:3px dashed red;"
|
|-
|-
! Supplied in the Kit
| Supplied in Kit||||    Amount
! Amount
|-
|-
| 10μM Forward Primer
| 10 μM Forward Primer ||||    2.5 μL
| 25μL
|-
|-
| 10μM Reverse Primer
| 10 μM Reverse Primer ||||    2.5 μL
| 2.5μL
|-
|-
| GoTaq Master Mix
| GoTaq master mix ||||    119.5 μL
| 125μL
|-
|-
| H<sub>2</sub>O
| dH<sub>2</sub>O ||||    119.5 μL
| 119.5μL
|-
|-
| Total Volume
| Total Volume ||||    250 μL
| 250μL
|}
|}



Revision as of 20:10, 26 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in Kit Amount
10 μM Forward Primer 2.5 μL
10 μM Reverse Primer 2.5 μL
GoTaq master mix 119.5 μL
dH2O 119.5 μL
Total Volume 250 μL


Suplied by User Amount
Sample of DNA by user (20ng) .5μL



PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration