BME103:W930 Group9: Difference between revisions
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[[Image:BME103 Group9 fluorimeter.png|300px|]]<br> | [[Image:BME103 Group9 fluorimeter.png|300px|]]<br> | ||
Fluorimeter setup <br><br> | Fluorimeter setup <br><br> | ||
Flourimeter procedure<br><br> | |||
1. place the flourimeter on the table and turn on the blue light.<br> | |||
2. place provided glass slide on flourimeter track so that the first row of dots is even with the light.<br> | |||
3. place phone cradle in front of the flourimeter with a smart phone facing perpendicular to the beam of light. (as seen in the picture). <br> | |||
4. add two drops of green dye on the dots that are even with the light.<br> | |||
5. Place two drops of DNA sample on top of the green dye.<br> | |||
6. cover the flourimeter and phone by turning the large box over and placing it above both of them.<br> | |||
7. Take a picture of the droplet with the camera.<br> | |||
8. save the picture and send it to the imageJ operator.<br> | |||
Instructions for opening images in imageJ<br><br> | Instructions for opening images in imageJ<br><br> | ||
1. | 1. Take a picture of the fluorimeter assembly with a smartphone.<br> | ||
2. | 2. Transfer the picture to a laptop equipped with imageJ via icloud or email.<br> | ||
3. | 3. Open imageJ and select file, then open.<br> | ||
4. | 4. Find the file on the computer and select it.<br> | ||
5. | 5. The image is now open and can be analyzed.<br> | ||
6. The image | 6. The image can be split into three images (blue, green, and red) for better analysis by selecting: image-color-split channels. | ||
Revision as of 23:02, 13 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design The open PCR is a device that enables the splitting of DNA. It is run through many cycles of heating and cooling that enables the splitting and recombination with polomers. It connects to a computer program to run the cycles. The temperature change is controled by the heating lid and than the information is fed to the LED screen. The heating lid heats tubes that are held in the heat tube holder to the appropriate temperature and then cools them as needed in each cycle. Experimenting With the Connections When we unplugged part 3, the LCD, from part 6, the Open PCR Brains Board, the LCD on the machine stopped working and did not show anything on it. When we unplugged the white wire that connects part 6, the Open PCR Brains Board, to part 2, the heat tube, the machine no longer measures the temperature of the plate and sends it to the LCD.
First test run: 24 Oct. 2012
ProtocolsPolymerase Chain Reaction Procedure for amplifying a person’s DNA
The eight Samples
Flourimeter procedure 1. place the flourimeter on the table and turn on the blue light. Instructions for opening images in imageJ
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology This report used the technique called DNA amplification. (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
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