BME103:W930 Group9: Difference between revisions
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* '''Sample''' = A sample was two drops of a flourescent dye that stains DNA green and two drops of DNA solution, either provided as a control, or obtained from open pcr. | * '''Sample''' = A sample was two drops of a flourescent dye that stains DNA green and two drops of DNA solution, either provided as a control, or obtained from open pcr. | ||
* '''Integrated Density''' = | * '''Integrated Density''' = Integrated density basically measures the amount of brightness there is in a certain area. This is an extensive property (one that depends on how many pixels were in the system) so areas as close in size to each other were used as measurements. The integrated density shown in the tablewas determined by the following means. The integrated density of an area immediately above the droplet was subtracted from the integrated density of the area covering the droplet. | ||
* '''DNA μg/mL''' = This value was calculated by dividing the integrated density of the sample by the integrated density of the positive control and multiplying that number by 2 μg/mL (the amount of DNA in the positive control). | * '''DNA μg/mL''' = This value was calculated by dividing the integrated density of the sample by the integrated density of the positive control and multiplying that number by 2 μg/mL (the amount of DNA in the positive control). | ||
* '''Conclusion''' = Samples were considered positive for the gene if they contained more than 1 μg/mL of DNA. Samples within .1 μg/mL were arbitrarily determined to be borderline, or tentatively positive. | * '''Conclusion''' = Samples were considered positive for the gene if they contained more than 1 μg/mL of DNA. Samples within .1 μg/mL were arbitrarily determined to be borderline, or tentatively positive. |
Revision as of 00:10, 14 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design The open PCR is a device that enables the splitting of DNA. It is run through many cycles of heating and cooling that enables the splitting and recombination with polomers. It connects to a computer program to run the cycles. The temperature change is controled by the heating lid and than the information is fed to the LED screen. The heating lid heats tubes that are held in the heat tube holder to the appropriate temperature and then cools them as needed in each cycle. Experimenting With the Connections When we unplugged part 3, the LCD, from part 6, the Open PCR Brains Board, the LCD on the machine stopped working and did not show anything on it. When we unplugged the white wire that connects part 6, the Open PCR Brains Board, to part 2, the heat tube, the machine no longer measures the temperature of the plate and sends it to the LCD.
First test run: 24 Oct. 2012
ProtocolsPolymerase Chain Reaction Procedure for amplifying a person’s DNA
The Samples
Flourimeter procedure 1. Place the flourimeter on the table and turn on the blue light. Instructions for opening images in imageJ
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology This report used the technique called DNA amplification. (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
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