BME103 s2013:T900 Group1: Difference between revisions
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'''Stage 3'''<br> | '''Stage 3'''<br> | ||
Final Hold 4°C for 3 minutes: ''PCR reaction is stopped.''<br><br> | Final Hold 4°C for 3 minutes: ''PCR reaction is stopped.''<br><br> | ||
[[Image:BME103 Group1 PCR Screenshot.jpg|450px|Screen shot of the Open PCR program detailed above]] | |||
'''DNA Sample Set-up'''<br> | '''DNA Sample Set-up'''<br> | ||
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'''PCR Reaction Mix'''<br> | '''PCR Reaction Mix'''<br> | ||
* Taq DNA polymerase | * Taq DNA polymerase | ||
* MgCl<sub>2</sub> | * MgCl<sub>2</sub> | ||
* dNTP's | * dNTP's | ||
'''DNA Sample''' | '''DNA Sample/Primer Mix'''<br> | ||
* Extracted sample of a particular patient's DNA | * Extracted sample of a particular patient's DNA | ||
* Forward Primer | |||
* Reverse Primer | |||
<br><br> | <br><br> |
Revision as of 15:59, 25 March 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPCR, or Polymerase Chain Reaction, is a method of amplifying a particular segment of DNA by way of selective replication. If the sequence of the target DNA segment is known, primers can be created from the complimentary base pairs at each end of the target segment. A forward as well as a reverse primer is required so that each double strand of DNA results in two double strands of DNA at the end of each cycle (as Taq DNA Polymerase only works in one direction, it needs a primer at the beginning of both strands in order to copy both). To complete PCR, a DNA sample (containing the target DNA segment) must be mixed with the primers (both forward and reverse as discussed above), Taq DNA Polymerase, dNTP's (free base pairs to be used as the building blocks in the new DNA put together by the polymerase), and MgCl2 (a required substrate for the polymerase to use the dNTP's). Stage 1
Stage 3
DNA Sample Set-up
DNA Sample Set-up Procedure
PCR Reaction Mix
DNA Sample/Primer Mix
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 9's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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