BME103 s2013:T900 Group1 L2: Difference between revisions
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'''Smart Phone Camera Settings'''<br> | '''Smart Phone Camera Settings'''<br> | ||
* We used an | * We used an iPhone 5 | ||
** Flash:Off | ** Flash:Off | ||
** ISO setting:NA | ** ISO setting:NA | ||
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* No additional phone used | * No additional phone used | ||
* A time delay setting was used, allowing 5 seconds for the light box to be closed before an image was captured. | |||
'''Calibration'''<br> | '''Calibration'''<br> | ||
In order to measure unknown concentrations of DNA with the fluorimeter by measuring fluorescence, we must first measure the fluorescence of known concentrations of DNA using the same technique. To accomplish this, we used an assay of several known concentrations of calf Thymus DNA in conjunction with SYBR green dye. We took pictures of the fluorescing drops and used imaging software to measure the fluorescence. SYBR green dye fluoresces green and the jimage software allows the colors of one picture to be split into pictures of the component colors. We can then select just the green light and measure the pixel density, allowing us to quantify the fluorescence. With a known concentration and a known pixel density, we can then define a relationship between the two which will allow us to measure the fluorescence of an unknown sample to determine the DNA concentration of it. <br> | |||
We inserted the | |||
We inserted the iPhone inside a cradle that gave us a right angle view. Afterward we adjusted the height of the phone so that it is as even with the drop. | |||
[[Image:Photo(1).JPG]] | [[Image:Photo(1).JPG]] | ||
* Distance between the smart phone cradle and drop = 3cm | * Distance between the smart phone cradle and drop = 3cm | ||
Revision as of 20:43, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter
ProcedureSmart Phone Camera Settings
Calibration
Data AnalysisRepresentative Images of Samples
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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