BME103 s2013:T900 Group1 L2: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
* ''Align light between first two clean rows of spots so it will shine directly at the drop, secure'' | * ''Align light between first two clean rows of spots so it will shine directly at the drop, secure slide with tightening screw'' | ||
* ''Using a clean micro-pipette tip, slowly place 80μL of SYBR green dye allowing it to span between both spots'' | * ''Using a clean micro-pipette tip, slowly place 80μL of SYBR green dye allowing it to span between both spots'' | ||
* ''Using a clean micro-pipette tip, add 80μL of the sample solution to the SYBR dye'' | * ''Using a clean micro-pipette tip, add 80μL of the sample solution to the SYBR dye'' | ||
* ''Complete assembly by placing the camera/cradle in position and cover with light box'' | * ''Complete assembly by placing the camera/cradle in position and cover with light box'' | ||
* ''Start timed delay on phone camera'' | * ''Start timed delay picture on phone camera'' | ||
* ''Close light box quickly and carefully | * ''Close light box quickly and carefully, then wait the allotted time for the picture to complete'' | ||
* ''Extract and discard solution from the slide'' | * ''Extract and discard solution from the slide'' | ||
These steps were repeated in triplicate for each of the samples listed above. | These steps were repeated in triplicate for each of the samples listed above. |
Revision as of 21:03, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter
ProcedureSmart Phone Camera Settings
Calibration
These steps were repeated in triplicate for each of the samples listed above.
Data AnalysisRepresentative Images of Samples
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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