OUR TEAM
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LAB 2 WRITE-UP
Background Information
SYBR Green Dye
[A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
- We used an Iphone
- Flash:None
- ISO setting:Auto
- White Balance:Auto
- Exposure:Auto
- Saturation:Auto
- Contrast:Auto
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
- Distance between the smart phone cradle and drop =
[See worksheet page 5]
Solutions Used for Calibration [See worksheet page 5]
row 1 cell 1 |
row 1 cell 2 |
row 1 cell 3 |
row 1 cell 4
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row 2 cell 1 |
row 2 cell 2 |
row 2 cell 3 |
row 2 cell 4
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row 3 cell 1 |
row 3 cell 2 |
row 3 cell 3 |
row 3 cell 4
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[Add more rows as needed]
Placing Samples onto the Fluorimeter
- [Step one, in your OWN words]
- [Step two, in your own words]
- [Step three, in your own words]
- [etc.]
Data Analysis
Representative Images of Samples
Image J Values for All Samples [See worksheet page 5]
Concentration |
Integrated Density |
Integrated Density of Background |
Area of Circle |
Mean Gray Value
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Water |
213454 |
?? |
6691 |
31.902
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Water |
93371 |
?? |
2422 |
38.551
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Water |
65949 |
?? |
1219 |
54.101
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0.25 |
593913 |
?? |
7333 |
80.992
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0.25 |
569386 |
?? |
6988 |
81.481
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0.25 |
791593 |
?? |
8917 |
88.773
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0.5 |
651140 |
?? |
7774 |
83.759
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0.5 |
453045 |
?? |
5146 |
88.038
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0.5 |
2142033 |
?? |
15458 |
138.571
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1 |
2458883 |
?? |
12175 |
201.962
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1 |
1836054 |
?? |
9380 |
195.741
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1 |
2223630 |
?? |
11335 |
196.174
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2 |
1721563 |
?? |
6868 |
250.664
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2 |
3215604 |
?? |
12780 |
251.612
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2 |
2577701 |
?? |
10205 |
252.592
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5 |
3957423 |
?? |
15704 |
252.001
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5 |
4194978 |
?? |
16748 |
250.476
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5 |
5088859 |
?? |
20200 |
251.924
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[Add more rows as needed]
Fitting a Straight Line
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