SYBR Green Dye
SYBR Green dye is a fluorescent dye that binds to double-stranded DNA, but will stain single-stranded DNA with lower performance.
Single-Drop Fluorimeter
Fluorimeter is a device that is used to measure the amount fluorescence in a single drop.
How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
We used an Iphone 5
Flash:Off
ISO setting:NA
White Balance:NA
Exposure:NA
Saturation:NA
Contrast:NA
No additional phone used
Calibration
We inserted the Iphone inside a cradle that gave us a right angle view. Afterward we adjusted the height of the phone so that it is as even with the drop.
Distance between the smart phone cradle and drop = 3cm
Solutions Used for Calibration[See worksheet page 5]
Calf Thymus DNA solution (microg/mL)
Volume of DNA Solution (μL)
Volume of SYBR GREEN I Dye solution (μL)
Final DNA concentration in PicoGreen Assay (ng/mL)
0
80
80
blank
.25
80
80
.125
.5
80
80
.25
1
80
80
.5
2
80
80
1
5
80
80
2.5
Placing Samples onto the Fluorimeter
[Step one, in your OWN words]
[Step two, in your own words]
[Step three, in your own words]
[etc.]
Data Analysis
Representative Images of Samples
Image J Values for All Samples[See worksheet page 5]