BME103 s2013:T900 Group2 L2: Difference between revisions
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| [[Image:404005_4622115081677_1186647743_n.jpg|100px|thumb|Mitch Riggs <br> Open PCR Machine Engineer/ Team Leader]] | | [[Image:404005_4622115081677_1186647743_n.jpg|100px|thumb|Mitch Riggs <br> Open PCR Machine Engineer/ Team Leader]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: William Scott]] | | [[Image:BME103student.jpg|100px|thumb|Name: William Scott]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Joe Sansone]] | | [[Image:BME103student.jpg|100px|thumb|Name: Joe Sansone <br> Protocol Specialist]] | ||
| [[Image: | | [[Image:Photo(3).jpg|100px|thumb|Name: Shang Ruan <br> Open PCR Machine Engineer]] | ||
|} | |} | ||
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'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
'' | ''The single drop fluorimeter emits a blue light at the drop of SYBR Green which will fluoresce green if it contains DNA. http://openwetware.org/images/c/c7/2012-11-01_11.53.50.jpg]''<br> | ||
When using it, put your thumb on the button, push it all the way down to squeeze the existed air in it and then collect the SYBR Green. A little plastic tip is used during the liquid collecting so the liquid in different tubes would not mess up with each other. There is a bar for pressing to eject the tip. Do not use fingers to pull it out. <br> | |||
'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> | ||
'' | ''A drop of DNA and SYBR Green is collected on a glass slide. A fluorometer is used to shine a blue light on the drop. The drop will fluoresce green light if enough DNA is in the solution. By measuring the amount of green light emitted the amount of DNA in the sample can be determined. Basically, fluorescence is the property of some atoms to absorb some light at a particular wavelength and to subsequently emit light of longer wavelength after a brief interval, it is like choosing students for the admission of the university. The Fluorescence technique is always used to examine or analyze protein-nucleic acid and protein-protein etc.'' | ||
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'''Calibration'''<br> | '''Calibration'''<br> | ||
''The camera was placed on a cradle that was in approximately equal height of the fluorimeter. If the cradle and camera needed to be taller in order to be of equal height to the fluorimeter, the cradle was then placed on a stacked glass case until the camera was parallel. The distance from the cradle to the fluorimeter was about 7 cm. After creating a solution for calibration the camera was then set on a self-timer of 10 seconds and then the fluorimeter was encased in a box and covered for complete darkness for the most accurate results. After the beep indicating that the picture was taken the cycle was then complete and the process was then repeated for each solution. '' | ''The camera was placed on a cradle that was in approximately equal height of the fluorimeter. If the cradle and camera needed to be taller in order to be of equal height to the fluorimeter, the cradle was then placed on a stacked glass case until the camera was parallel. The distance from the cradle to the fluorimeter was about 7 cm. After creating a solution for calibration the camera was then set on a self-timer of 10 seconds and then the fluorimeter was encased in a box and covered for complete darkness for the most accurate results. After the beep indicating that the picture was taken the cycle was then complete and the process was then repeated for each solution. | ||
Image of setup as shown from http://openwetware.org/wiki/BME103'' | |||
<font color="red">***PHOTO REMOVED DUE TO PLAGIARISM*** Original photos from other lab reports should not be reused.</font> | |||
* Distance between the smart phone cradle and drop = '''7cm''' | * Distance between the smart phone cradle and drop = '''7cm''' | ||
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'''Representative Images of Samples''' | '''Representative Images of Samples''' | ||
''[ | ''[Water Droplet (control/blank) http://openwetware.org/images/6/6e/Water_droplet_blank.jpg]'' | ||
''[ | ''[High Concentrated DNA Solution Drop with SYBR Dye http://openwetware.org/images/0/06/High_concentration_DNA_drop.jpg]'' | ||
'''Image J Values for All Samples''' | '''Image J Values for All Samples''''' | ||
{| {{table}} width=700 | {| {{table}} width=700 | ||
|- | |- | ||
| | | DNA final concentration (ng/mL)|| Ave. Mean|| Ave. IntDen | ||
|- | |||
| 2.5 || 109.55 || 4920177 | |||
|- | |||
| 1 || 98.36 || 4687729 | |||
|- | |||
| 0.5 || 58.41 || 2081008 | |||
|- | |||
| 0.25|| 49.45 || 1679326 | |||
|- | |- | ||
| | | 0.125 || 47.1 || 1396192 | ||
|- | |- | ||
| | | 0 || 35.93 || 1195111 | ||
|} | |} | ||
'''Fitting a Straight Line'''<br> | '''Fitting a Straight Line'''<br> | ||
''[ | ''[ Integrated Density of DNA Droplets with SYBR Dye http://openwetware.org/images/7/71/Line_of_best_fit.jpg]'' | ||
Latest revision as of 08:55, 28 June 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Calibration The camera was placed on a cradle that was in approximately equal height of the fluorimeter. If the cradle and camera needed to be taller in order to be of equal height to the fluorimeter, the cradle was then placed on a stacked glass case until the camera was parallel. The distance from the cradle to the fluorimeter was about 7 cm. After creating a solution for calibration the camera was then set on a self-timer of 10 seconds and then the fluorimeter was encased in a box and covered for complete darkness for the most accurate results. After the beep indicating that the picture was taken the cycle was then complete and the process was then repeated for each solution. Image of setup as shown from http://openwetware.org/wiki/BME103 ***PHOTO REMOVED DUE TO PLAGIARISM*** Original photos from other lab reports should not be reused.
Solutions Used for Calibration
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Water Droplet (control/blank) http://openwetware.org/images/6/6e/Water_droplet_blank.jpg] [High Concentrated DNA Solution Drop with SYBR Dye http://openwetware.org/images/0/06/High_concentration_DNA_drop.jpg]
Image J Values for All Samples
Fitting a Straight Line
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