BME103 s2013:T900 Group2 L2: Difference between revisions
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| Calf Thymus DNA Solution (microg/mL) || Volume 2X DNA Solution (uL) || Volume SYBR GREEN I Solution (uL)|| Fina DNA concentration in PicoGreen Assay (ng/mL) | | Calf Thymus DNA Solution (microg/mL) || Volume 2X DNA Solution (uL) || Volume SYBR GREEN I Solution (uL)|| Fina DNA concentration in PicoGreen Assay (ng/mL) | ||
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| 5 || | | 5 || 80 || 80 || 2.5 | ||
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| 2 || | | 2 || 80 || 80 || 1 | ||
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| 1|| | | 1|| 80|| 80|| 0.5 | ||
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| 0.5 || | | 0.5 || 80|| 80 || 0.25 | ||
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| 0.25 || | | 0.25 || 80 || 80|| 0.125 | ||
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| 0 || | | 0 || 80 || 80|| blank | ||
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Revision as of 15:24, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[See worksheet page 5]
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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