BME103 s2013:T900 Group2 L2

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(Procedure)
(Procedure)
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''[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''
''[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''
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* Distance between the smart phone cradle and drop =
+
* Distance between the smart phone cradle and drop = '''7cm'''
''[See worksheet page 5]''
''[See worksheet page 5]''
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{| {{table}} width=700
{| {{table}} width=700
|-
|-
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| Calf Thymus DNA Solution (microg/mL) || Volume 2X DNA Solution (uL) || Volume SYBR GREEN I Solution (uL)|| Fina DNA concentration in PicoGreen Assay (ng/mL)
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| '''Calf Thymus DNA Solution (microg/mL)''' || '''Volume 2X DNA Solution (uL)''' || '''Volume SYBR GREEN I Solution (uL)'''|| '''Fina DNA concentration in PicoGreen Assay (ng/mL)'''
|-
|-
| 5 || 80 || 80 || 2.5
| 5 || 80 || 80 || 2.5

Revision as of 17:30, 1 April 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: Andy Son
Name: Andy Son
Mitch Riggs  Open PCR Machine Engineer/ Team Leader
Mitch Riggs
Open PCR Machine Engineer/ Team Leader
Name: William Scott
Name: William Scott
Name: Joe Sansone
Name: Joe Sansone
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
SYBR Green dye is a dye used as an indicator in molecular biology. It emits green light when bound to double helix DNA and illuminated with blue light. Source: http://en.wikipedia.org/wiki/SYBR_Green_I


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop = 7cm

[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

Calf Thymus DNA Solution (microg/mL) Volume 2X DNA Solution (uL) Volume SYBR GREEN I Solution (uL) Fina DNA concentration in PicoGreen Assay (ng/mL)
5 80 80 2.5
2 80 80 1
1 80 80 0.5
0.5 80 80 0.25
0.25 80 80 0.125
0 80 80 blank


Placing Samples onto the Fluorimeter

  • [Step one, in your OWN words]
  • [Step two, in your own words]
  • [Step three, in your own words]
  • [etc.]


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]




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