BME103 s2013:T900 Group2 L2: Difference between revisions
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'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> | ||
''A drop of DNA and SYBR Green is collected on a glass slide. A fluorometer is used to shine a blue light on the drop. The drop will fluoresce green light if enough DNA is in the solution. | ''A drop of DNA and SYBR Green is collected on a glass slide. A fluorometer is used to shine a blue light on the drop. The drop will fluoresce green light if enough DNA is in the solution. By measuring the amount of green light emitted the amount of DNA in the sample can be determined. Basically, fluorescence is the property of some atoms to absorb some light at a particular wavelength and to subsequently emit light of longer wavelength after a brief interval, it is like choosing students for the admission of the university. The Fluorescence technique is always used to examine or analyze protein-nucleic acid and protein-protein etc.'' | ||
Revision as of 09:59, 2 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Calibration The camera was placed on a cradle that was in approximately equal height of the fluorimeter. If the cradle and camera needed to be taller in order to be of equal height to the fluorimeter, the cradle was then placed on a stacked glass case until the camera was parallel. The distance from the cradle to the fluorimeter was about 7 cm. After creating a solution for calibration the camera was then set on a self-timer of 10 seconds and then the fluorimeter was encased in a box and covered for complete darkness for the most accurate results. After the beep indicating that the picture was taken the cycle was then complete and the process was then repeated for each solution. Image of setup as shown from http://openwetware.org/wiki/BME103 http://openwetware.org/images/e/e3/BME103_Group9_FluorimeterSetup.jpg
Solutions Used for Calibration
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Water Droplet (control/blank) http://openwetware.org/images/6/6e/Water_droplet_blank.jpg] [High Concentrated DNA Solution Drop with SYBR Dye http://openwetware.org/images/0/06/High_concentration_DNA_drop.jpg]
Image J Values for All Samples
Fitting a Straight Line
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