BME103 s2013:T900 Group4: Difference between revisions
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'''The Original Design'''<br> | '''The Original Design'''<br> | ||
(Add image of the full OpenPCR machine here | (Add image of the full OpenPCR machine here) | ||
Here's the OpenPCR machine. Made up of thin pieces of wood, the case contains the hardware of the machine. With another smaller wood case on top of the main case containing the loading bay which acts as a lid. Small test vials can be placed into holders here in the bay to be heated by PCR machine. Inside the main body of the machine sits the hardware similar to a personal computer. A processor unit connects to the heating hardware as well as the power supply and led display board. Above the power supply lies the heatsink with a fan attached facing vents in the case allowing for efficient thermal control inside the machine to prevent overheating. The machine then has sockets that allow the machine to be hooked up by USB to a computer. Using a computer the PCR can be ordered to heat up the vials in the heating lid on certain intervals at different temperatures as well as indicated cycles.<br> | Here's the OpenPCR machine. Made up of thin pieces of wood, the case contains the hardware of the machine. With another smaller wood case on top of the main case containing the loading bay which acts as a lid. Small test vials can be placed into holders here in the bay to be heated by PCR machine. Inside the main body of the machine sits the hardware similar to a personal computer. A processor unit connects to the heating hardware as well as the power supply and led display board. Above the power supply lies the heatsink with a fan attached facing vents in the case allowing for efficient thermal control inside the machine to prevent overheating. The machine then has sockets that allow the machine to be hooked up by USB to a computer. Using a computer the PCR can be ordered to heat up the vials in the heating lid on certain intervals at different temperatures as well as indicated cycles.<br> | ||
Revision as of 00:07, 26 March 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program DNA Sample, 50 μL each: patient ID's from the UnderGrad Teaching Assistant(UGTA).
DNA Sample Set-up Procedure
DNA/ primer mix
Research and DevelopmentMixture components: DNA Template (Sample DNA) Procedure: Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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