BME103 s2013:T900 Group4

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student: Kinjal Ahir Role(Experimental protocol planner) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Sample, 50 μL each: patient ID's from the UnderGrad Teaching Assistant(UGTA). Positive control Patient 1 Patient 1 Patient1 cancer DNA template ID:85158 ID: 85158 ID: 85158 Replicate 1 Replicate 2 Replicate 3 Tube label: A+ Tube label: B1 Tube label: C1 Tube label: D1 Negative Control: no Patient 2 Patient 2 Patient 2 DNA template ID____17818_____ ID_____17818_______ ID______17818______ Replicate 1 Replicate 2 Replicate 3 Tube label___A-____ Tube label___B2____ Tube label__C2_____ Tube label__D2_____ DNA Sample Set-up Procedure Step 1: Student were Provided with 8 tubes of 50 μL PCR reaction mix, 8 tubes of 50 μL diluted template + primers, and disposable transfer pipettes. Step 2: Protocol planner labeled the test tube that is provided above. Step 3: 50μL of each DNA sample Mix in to test tube. Step 4: Protocol planner set up the DNA and ran the PCR reactions. PCR Reaction Mix In the PCR reaction mix there is Mix contains Taq DNA polymerase, MgCl2, dNTP's, forward primer, reverse primer. DNA/ primer mix In the DNA/ primer mix there is patient's DNA. Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)