BME103 s2013:T900 Group4
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program DNA Sample, 50 μL each: patient ID's from the UnderGrad Teaching Assistant(UGTA).
DNA Sample Set-up Procedure
DNA/ primer mix
Basic DNA components: DNA Template DNA Polymerase DNA Primers Nucleotides Procedure: First combine the components are combined together and heat it. Once the reaction is heated to a temperature of 95 degrees Celsius, the hydrogen bonds between the DNA strands break and create two separate DNA sequences. After that, allow it to cool down to a temperature of around 55 degrees Celsius. As it cools, the DNA primers are able to hybridize to the separate DNA strands. The DNA polymerase is going the bind the DNA sequence with the DNA Primer. Once, the DNA polymerase is bound, it is going to take some of the free nucleotides from the surrounding area and complete the DNA strand. The result is a two separate DNA sequences, with two DNA strand identical to the first one. Repeatedly doing the steps and the result amount of DNA stands multiplied exponentially.
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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