BME103 s2013:T900 Group4

From OpenWetWare

Revision as of 15:33, 24 March 2013 by Kinjal Ahir (Talk | contribs)
Jump to: navigation, search
BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: student: Kinjal Ahir Role(Experimental protocol planner)
Name: student: Kinjal Ahir Role(Experimental protocol planner)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Thermal Cycler Program

DNA Sample, 50 μL each: patient ID's from the UnderGrad Teaching Assistant(UGTA).

Positive control Patient 1 Patient 1 Patient1
cancer DNA template ID____________ ID____________ ID_____________
Replicate 1 Replicate 2 Replicate 3
Tube label_______ Tube label_______ Tube label_______ Tube label_______
Negative Control: no Patient 2 Patient 2 Patient 2
DNA template ID_________ ID____________ ID____________
Replicate 1 Replicate 2 Replicate 3
Tube label_______ Tube label_______ Tube label_______ Tube label_______

DNA Sample Set-up Procedure

  1. Step 1: Student were Provided with 8 tubes of 50 μL PCR reaction mix, 8 tubes of 50 μL diluted template + primers, and disposable transfer pipettes.
  2. Step 2: The instructors were provided to test positive and negative for a DNA marker.
  3. Step 3: Protocol planner set up the DNA and ran the PCR reactions.


PCR Reaction Mix

  • What is in the PCR reaction mix?

DNA/ primer mix

  • What is in the DNA/ primer mix?






Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)





Personal tools