BME103 s2013:T900 Group4 L2: Difference between revisions
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'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
SYBR Green I is a fluorescent dye that binds to double stranded DNA. This dye then fluoresces green when excited by blue light. SYBR green dye is very efficient at visualizing DNA, incredibly safe to users, and cheap, making it a prime dye for basic DNA displaying. | |||
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SYBR Green I is a fluorescent dye that | |||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
A fluorimeter is a device used to measure parameters of fluorescence concentration. The device usually measures fixed wavelengths either by filters, monochromators or fixed diodes. Fluorometers have two light detectors: one light detects the absorbance, while the other detects fluorescence emission. <br> | |||
'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> | ||
The fluorescence technique uses flourescence to measure the concentration of substrates in mixed liquids. This is done by using a single drop fluorometer with a Teflon-coated glass slide. The slide's surface is not completely covered; circles of bare glass remain, which water sticks to, allowing drops of liquids to stay in place. Drops of DNA solution are placed on the glass circles along with SYBR Green I. Blue LED light from the fluorometer is aimed at the drops, exciting the dye and causing it to fluoresce. | |||
Revision as of 00:18, 2 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Photo of Fluorimeter
Data AnalysisRepresentative Images of Samples
Circle Analysis of DNA positive drop. 5 concentration.
As shown, the DNA positive drop is much more illuminated.
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