BME103 s2013:T900 Group4 L3: Difference between revisions
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<!-- If your goal includes modifying the Fluorimeter, include a labeled image or images of the Fluorimeter. There is no Solid Works file for the Fluorimeter. Write a short paragraph that summarizes what your team is going to modify --> | <!-- If your goal includes modifying the Fluorimeter, include a labeled image or images of the Fluorimeter. There is no Solid Works file for the Fluorimeter. Write a short paragraph that summarizes what your team is going to modify --> | ||
<!-- If your goal does not require any changes in the machinery/ devices, include labeled images of the OpenPCR machine and the Fluorimeter. Write a short paragraph that summarizes what each one of the machines does --> | <!-- If your goal does not require any changes in the machinery/ devices, include labeled images of the OpenPCR machine and the Fluorimeter. Write a short paragraph that summarizes what each one of the machines does --> | ||
We decided to have some modification of the fluorimeter to allow easy adjustment and positioning of the phone. A base or a phone holder will be made to be able to connect to the fluorimeter. This will allow the phone to be held in place. The base will have option to set the length, height, and distance of the phone to the fluorimeter, and should be compatible with most camera phones/PDA on the market to be used. The benefit of this modification is that it will help save time to try to readjust the phone in the correct position, and that photos can be taken better and accurate, which give better and accurate data results. | |||
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<!-- If your team decided not to change any of the machinery/ devices, write a short step-by-step list of instructions on how to set up and use each machine. You may want to consider modifying the instructions to improve ease of use. --> | <!-- If your team decided not to change any of the machinery/ devices, write a short step-by-step list of instructions on how to set up and use each machine. You may want to consider modifying the instructions to improve ease of use. --> | ||
Step 1: Connect the base to the fluorimeter. | |||
Step 2: Set the camera phone into the base. | |||
Step 3: Set the height and distance. | |||
Step 4: Take Photo. | |||
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==New System: Protocols== | ==New System: Protocols== |
Revision as of 22:43, 15 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 3 WRITE-UPOriginal System: PCR ResultsPCR Test Results
* Ave. INTDEN = Average of ImageJ integrated density values from three Fluorimeter images
Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)
Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.
New System: Design StrategyWe concluded that a good system Must Have:
- Result easy to determine. - Simple OpenPCR Software: The OpenPCR Software shoulder be simple and easy to use and that would help lab experiments done faster, rather than the need to spend extra time to figure out how the software works. We concluded that we would Want a good system to have:
- Low cost: OpenPCR $599 Fluorimeter $300. A low cost material would help since there is always a limited budge for the research experiment. This would allow us to buy more OpenPCR and fluorimeter and create more data results in the same amount of time. Phone camera should not be easily moveable when played in front of the fluorimeter. Phone camera adjustment can be time consuming and not setting it in the correct position/distance results data error. There should be an extra modification where the camera should be in a fixed position and distance and easily re-adjustable.
- Troublesome USB Connectivity. USB connectivity should function well in order for OpenPCR machine to work. - Easy to mix drops during imaging.
- Avoid slow amplification. - Hard to adjust phone/ fluorimeter. The phone can be easily moved by accident, which requires readjustment between the phone and the fluorimeter.
New System: Machine/ Device EngineeringSYSTEM DESIGN
We chose to include these new features
[OR] We chose keep the devices the same as the original system
INSTRUCTIONS Step 1: Connect the base to the fluorimeter. Step 2: Set the camera phone into the base. Step 3: Set the height and distance. Step 4: Take Photo.
New System: ProtocolsDESIGN We chose to include these new approaches/ features
[OR] We chose keep the protocols the same as the original system
New System: Research and DevelopmentBACKGROUND
DESIGN
New System: Software[THIS SECTION IS OPTIONAL. If your team has creative ideas for new software, and new software is a key component included in your new protocols, R&D, or machine design, you may describe it here. You will not receive bonus points, but a solid effort may raise your overall page layout points. If you decide not to propose new software, please delete this entire section, including the ==New System: Software== header.]
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