BME103 s2013:T900 Group5 L2: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
* Step 1.) With the Camera set in place we prepared the slide with SYBR Green I, a constant throughout the experiment <br> | * Step 1.) With the Camera set in place we prepared the slide with SYBR Green I, a constant throughout the experiment <br> | ||
* Step 2.) 80 μL of | * Step 2.) 80 μL of 5μg/mL of calf thymus concentration DNA samples were then added to the slide <br> | ||
* | * Step 3.) To take 3 pictures in the the dark with as much of the blue LED light as possible, we used a timer on the camera, set to 3 seconds. <br> | ||
* | * Step 4.) The slide, now containing SYBER Green I and Calf Thymus DNA was then aligned in the cradle such that the light was shining into the DNA. <br> | ||
* | * Step 5.) With the Camera phone, we captured 3 images of the DNA and removed the light box. <br> | ||
* | * Step 6.) That sample was then disposed of with a micropipette <br> | ||
* Step 7.) Repeat with 80μL Green SABR I and 80μL of each Calf Thymus Concentration given by the first column in the table above<br> | |||
<br> | |||
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Revision as of 22:27, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[See worksheet page 5]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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