BME103 s2013:T900 Group5 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Alexander Oropel Name: Matt McClintock Name: student Name: student Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye [A short summary describing SYBR green dye] Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points] How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Settings [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Calibration [Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = [See worksheet page 5] Solutions Used for Calibration [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 [Add more rows as needed] Placing Samples onto the Fluorimeter Step 1.) With the Camera set in place we prepared the slide with SYBR Green I, a constant throughout the experiment Step 2.) 80 μL of 5μg/mL of calf thymus concentration DNA samples were then added to the slide Step 3.) To take 3 pictures in the the dark with as much of the blue LED light as possible, we used a timer on the camera, set to 3 seconds. Step 4.) The slide, now containing SYBER Green I and Calf Thymus DNA was then aligned in the cradle such that the light was shining into the DNA. Step 5.) With the Camera phone, we captured 3 images of the DNA and removed the light box. Step 6.) That sample was then disposed of with a micropipette Step 7.) Repeat with 80μL Green SABR I and 80μL of each Calf Thymus Concentration given by the first column in the table above Data Analysis Representative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5 [Add more rows as needed] Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]