BME103 s2013:T900 Group5 L2

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(Procedure)
(Procedure)
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'''Solutions Used for Calibration''' ''[See worksheet page 5]''
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'''Solutions Used for Calibration'''  
{| {{table}} width=700
{| {{table}} width=700
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| Concentration Calf Thymus <br> (μg/mL) || Volume DNA added <br> (μL)|| Volume SABR GREEN I (μL) || Concentration resulting DNA ng/mL
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| "Concentration Calf Thymus" <br> "(μg/mL)" || "Volume DNA added" <br> "(μL)"|| "Volume SABR GREEN I" <br> "(μL)" || "Concentration resulting DNA"<br> "(ng/mL)"
|-
|-
| 5 || 80 || 80 || 2.5
| 5 || 80 || 80 || 2.5

Revision as of 01:41, 2 April 2013

BME 103 Spring 2013 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: Alexander Oropel
Name: Alexander Oropel
Name: Matt McClintock
Name: Matt McClintock
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[See worksheet page 5]


Solutions Used for Calibration

"Concentration Calf Thymus"
"(μg/mL)"
"Volume DNA added"
"(μL)"
"Volume SABR GREEN I"
"(μL)"
"Concentration resulting DNA"
"(ng/mL)"
5 80 80 2.5
2 80 80 1
1 80 80 0.5
0.5 80 80 0.25
0.25 80 80 .125
0 80 80 blank


Placing Samples onto the Fluorimeter

  • Step 1.) With the Camera set in place we prepared the slide with SYBR Green I, a constant throughout the experiment
  • Step 2.) 80 μL of 5μg/mL of calf thymus concentration DNA samples were then added to the slide
  • Step 3.) To take 3 pictures in the the dark with as much of the blue LED light as possible, we used a timer on the camera, set to 3 seconds.
  • Step 4.) The slide, now containing SYBER Green I and Calf Thymus DNA was then aligned in the cradle such that the light was shining into the DNA.
  • Step 5.) With the Camera phone, we captured 3 images of the DNA and removed the light box.
  • Step 6.) That sample was then disposed of with a micropipette
  • Step 7.) Repeat with 80μL Green SABR I and 80μL of each Calf Thymus Concentration given by the first column in the table above

Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

[Add more rows as needed]Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]
row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5
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