BME103 s2013:T900 Group6

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| Positive Control - Cancer || 1-1 || 1-2 || 1-3
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| Positive Control - Cancer || Patient 1- Vial 1 || Patient 1- Vial 2 || Patient 1- Vial 3
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| Negative Control - No Cancer || 2-1 || 2-2 || 2-3
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| Negative Control - No Cancer || Patient 2- Vial 1 || Patient 2- Vial 2 || Patient 2- Vial 3
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Revision as of 04:46, 24 March 2013

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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Contents

OUR TEAM

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LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Thermal Cycler Program

DNA Sample Set-up

Positive Control - Cancer Patient 1- Vial 1 Patient 1- Vial 2 Patient 1- Vial 3
Negative Control - No Cancer Patient 2- Vial 1 Patient 2- Vial 2 Patient 2- Vial 3

DNA Sample Set-up Procedure 1. Obtain DNA samples, PCR, DNA/Primer vials 2. Open All The Tops 3. Use pipette to move DNA into vials containing PCR mix

PCR Reaction Mix

  • What is in the PCR reaction mix?

The PCR mix is made up of 50 Microliters of PCR.

DNA/ primer mix

  • What is in the DNA/ primer mix?

The DNA/Primer Mix is made of 50 micro liters of DNA of patients 1 and 2, and primer. There are 2 vials containing negative and positive control. The negative control does not have cancer DNA, and the positive control has cancer in it.





Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)





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