BME103 s2013:T900 Group6

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(Protocols)
(Research and Development)
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==Research and Development==
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:==Protocols==
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'''Specific Cancer Marker Detection - The Underlying Technology'''<br>
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'''Thermal Cycler Program'''<br>
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(Add a write-up of the information discussed in Week 3's class)<br>
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'''DNA Sample Set-up'''<br>
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(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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{| {{table}}
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|-
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| Positive Control - Cancer || Patient 1- Vial 1 || Patient 1- Vial 2 || Patient 1- Vial 3
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|-
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| Negative Control - No Cancer || Patient 2- Vial 1 || Patient 2- Vial 2 || Patient 2- Vial 3
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|}
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'''DNA Sample Set-up Procedure'''
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1. Obtain DNA samples, PCR, DNA/Primer vials
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2. Open All The Tops
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3. Use pipette to move DNA into vials containing PCR mix
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<br><br>
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'''PCR Reaction Mix'''
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* What is in the PCR reaction mix?
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The PCR mix is made up of 50 Microliters of PCR.
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'''DNA/ primer mix'''
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* What is in the DNA/ primer mix?
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The DNA/Primer Mix is made of 50 micro liters of DNA of patients 1 and 2, and primer. There are 2 vials containing negative and positive control. The negative control does not have cancer DNA, and the positive control has cancer in it.
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Revision as of 03:43, 26 March 2013

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
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OUR TEAM

Name: Cyril Wassef-Initial Machine Tester
Name: Cyril Wassef
-Initial Machine Tester
Name: Israel BrewerResearch and Development
Name: Israel Brewer
Research and Development
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
Name: studentRole(s)
Name: student
Role(s)
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LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
Description: This is a PCR machine. It is a piece of equipment that allows scientists to create different amounts of DNA sequences. With it, scientists are able to denature (break down) specific DNA strands by inducing heat, and thus are able to analyze them specifically. This machine helps with research on possible DNA mutation, and aids in on-going research to help resolve today's problems with diseases such as cancer.

Image:PCRmachine.jpg


Experimenting With the Connections

When we unplugged the LCD screen from the OpenPCR circuit board, the machine's LED failed to work properly.

When we unplugged the white wire that connects the OpenPCR circuit board to the main heating block, the machine began to show a change of the temperature on the LCD screen.


Test Run

The date the PCR machine was open to conduct an analysis was on March 5th, 2013. We had machine number 6 that day. What we came to see, in terms of pros and cons, contained the following:

Pros: - Lightweight - Quiet - Ease of Use

Cons: - Took a while to complete the full cycle - The lid is not easy to remove. Requires some work - Requires a computer - Small and somewhat heavy - Made of Wood (fire hazard)




  • Dale Franco L. Caagbay:==Protocols==

Thermal Cycler Program

DNA Sample Set-up

Positive Control - Cancer Patient 1- Vial 1 Patient 1- Vial 2 Patient 1- Vial 3
Negative Control - No Cancer Patient 2- Vial 1 Patient 2- Vial 2 Patient 2- Vial 3

DNA Sample Set-up Procedure 1. Obtain DNA samples, PCR, DNA/Primer vials 2. Open All The Tops 3. Use pipette to move DNA into vials containing PCR mix

PCR Reaction Mix

  • What is in the PCR reaction mix?

The PCR mix is made up of 50 Microliters of PCR.

DNA/ primer mix

  • What is in the DNA/ primer mix?

The DNA/Primer Mix is made of 50 micro liters of DNA of patients 1 and 2, and primer. There are 2 vials containing negative and positive control. The negative control does not have cancer DNA, and the positive control has cancer in it.





==Protocols==

Thermal Cycler Program

DNA Sample Set-up

Positive Control - Cancer Patient 1- Vial 1 Patient 1- Vial 2 Patient 1- Vial 3
Negative Control - No Cancer Patient 2- Vial 1 Patient 2- Vial 2 Patient 2- Vial 3

DNA Sample Set-up Procedure 1. Obtain DNA samples, PCR, DNA/Primer vials 2. Open All The Tops 3. Use pipette to move DNA into vials containing PCR mix

PCR Reaction Mix

  • What is in the PCR reaction mix?

The PCR mix is made up of 50 Microliters of PCR.

DNA/ primer mix

  • What is in the DNA/ primer mix?

The DNA/Primer Mix is made of 50 micro liters of DNA of patients 1 and 2, and primer. There are 2 vials containing negative and positive control. The negative control does not have cancer DNA, and the positive control has cancer in it.





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