BME103 s2013:T900 Group6 L2: Difference between revisions
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| [[Image:BME103student.jpg|100px|thumb|Name: Dale Franco L. Caagbay - Protocol]] | | [[Image:BME103student.jpg|100px|thumb|Name: Dale Franco L. Caagbay - Protocol]] | ||
| [[Image:Labtwo.jpg|100px|thumb|Name: Cyril Wassef - Background Information]] | | [[Image:Labtwo.jpg|100px|thumb|Name: Cyril Wassef - Background Information/Data Analysis]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Emmanuel Casildo - Background Information]] | | [[Image:BME103student.jpg|100px|thumb|Name: Emmanuel Casildo - Background Information]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Israel Brewer]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student]] | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student]] | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
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Camera Phone <br> | Camera Phone <br> | ||
Dark Box <br> | Dark Box <br> | ||
SYBR Green 200mL <br> | SYBR Green 200mL <br> | ||
Flourimeter <br> | Flourimeter <br> | ||
DNA Sample Solution <br> | DNA Sample Solution <br> | ||
Micropipette and Tips <br> | Micropipette and Tips <br> | ||
Phone Cradle<br> | |||
'''Smart Phone Camera Settings'''<br> | '''Smart Phone Camera Settings'''<br> | ||
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'''Calibration'''<br> | '''Calibration'''<br> | ||
The iPhone was placed in a cradle in a way that made the lens the same height of the fluorimeter. In order to make them the same height, glass platforms were placed to raise the height of the fluorimeter. The distance was around 8 cm. The whole system was placed under the dark box and the pictures were taken. This was repeated for each new solution. | |||
Photo of Fluorimeter | Photo of Fluorimeter | ||
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'''Representative Images of Samples''' | '''Representative Images of Samples''' | ||
''[ | ''[[Image:before.jpg|400px|]]'' | ||
*Sample before DNA was present | |||
''[ | ''[[Image:after.jpg|400px|]]'' | ||
*Sample After DNA was present | |||
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{| {{table}} width=700 | {| {{table}} width=700 | ||
|- | |- | ||
| | | DNA Final Concentration (ng/mL)|| Average Mean|| Average IntDens | ||
|- | |||
| 5 || 106.75 || 4083499 | |||
|- | |||
| 2 || 95.35 || 4017729 | |||
|- | |||
| 1 || 79.58 || 3390288 | |||
|- | |||
| .5|| 54.68 || 2879054 | |||
|- | |- | ||
| | | .25 || 45.90 || 1859347 | ||
|- | |- | ||
| | | 0 || 33.69 || 994680 | ||
|} | |} | ||
'''Fitting a Straight Line'''<br> | '''Fitting a Straight Line'''<br> | ||
''[ | ''[[Image:bmelab2graph.jpg|600px|]]'' | ||
Latest revision as of 11:25, 2 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProtocolMaterials Smart Phone Camera Settings
Calibration The iPhone was placed in a cradle in a way that made the lens the same height of the fluorimeter. In order to make them the same height, glass platforms were placed to raise the height of the fluorimeter. The distance was around 8 cm. The whole system was placed under the dark box and the pictures were taken. This was repeated for each new solution. Photo of Fluorimeter
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples
Image J Values for All Samples [See worksheet page 5]
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