BME103 s2013:T900 Group6 L2

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| row 1 cell 1 || row 1 cell 2 || row 1 cell 3 || row 1 cell 4
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| Calf Thymus DNA solution concentration (microg/mL) || Volume of the 2X DNA solution (uL) || Volume of the SYBR GREEN I Dye Solution (uL) || Final DNA concentration in PicoGreen Assay (ng/mL)
|-
|-
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| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
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| 0 || 80 || 80 || blank
|-
|-
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| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
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| .25 || 80 || 80 || .125
 +
|-
 +
| .5 || 80 || 80 || .25
 +
|-
 +
| 1 || 80 || 80 || .5
 +
|-
 +
| 2 || 80 || 80 || 1
 +
|-
 +
| 5  || 80 || 80 || 2.5
|}
|}
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''[Add more rows as needed]''
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Revision as of 12:41, 26 March 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A PHOTO and description of the single-drop fluorimeter device]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]

  • Distance between the smart phone cradle and drop =

[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (uL) Volume of the SYBR GREEN I Dye Solution (uL) Final DNA concentration in PicoGreen Assay (ng/mL)
0 80 80 blank
.25 80 80 .125
.5 80 80 .25
1 80 80 .5
2 80 80 1
5 80 80 2.5


Placing Samples onto the Fluorimeter

  • [Step one, in your OWN words]
  • [Step two, in your own words]
  • [Step three, in your own words]
  • [etc.]


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]




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