BME103 s2013:T900 Group6 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Name: student Name: student Name: student Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye [A short summary describing SYBR green dye] Single-Drop Fluorimeter [A PHOTO and description of the single-drop fluorimeter device] How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Settings [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Calibration [Place a PHOTO that shows your camera phone set up in front of the flourimeter here] Distance between the smart phone cradle and drop = [See worksheet page 5] Solutions Used for Calibration [See worksheet page 5] Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (uL) Volume of the SYBR GREEN I Dye Solution (uL) Final DNA concentration in PicoGreen Assay (ng/mL) 0 80 80 blank .25 80 80 .125 .5 80 80 .25 1 80 80 .5 2 80 80 1 5 80 80 2.5 Placing Samples onto the Fluorimeter [Step one, in your OWN words] [Step two, in your own words] [Step three, in your own words] [etc.] Data Analysis Representative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5 [Add more rows as needed] Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]