BME103 s2013:T900 Group6 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Name: student Name: student Name: student Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye [A short summary describing SYBR green dye] Single-Drop Fluorimeter [A PHOTO and description of the single-drop fluorimeter device] How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Settings Iphone 5 Flash: None ISO setting: NA White Balance: NA Exposure: NA Saturation: NA Contrast: NA Calibration Photo of Fluorimeter File:IMAG0209 Distance between the smart phone cradle and drop = 8cm Solutions Used for Calibration Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (uL) Volume of the SYBR GREEN I Dye Solution (uL) Final DNA concentration in PicoGreen Assay (ng/mL) 0 80 80 blank .25 80 80 .125 .5 80 80 .25 1 80 80 .5 2 80 80 1 5 80 80 2.5 Placing Samples onto the Fluorimeter Get Slide, Micropipette and vial of sample Place slide into fluorimeter Take 80 micro liters of sample into micropipette Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice Make sure the drops form a ball and don't spread around individually Data Analysis Representative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5 [Add more rows as needed] Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]