BME103 s2013:T900 Group6 L2

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(Background Information)
(Procedure)
Line 89: Line 89:
* Place slide into fluorimeter
* Place slide into fluorimeter
* Take 80 micro liters of sample into micropipette
* Take 80 micro liters of sample into micropipette
-
* Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice
+
* Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice (place new drops in different area)
* Make sure the drops form a ball and don't spread around individually
* Make sure the drops form a ball and don't spread around individually
 +
* Clean Up Workstation
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Revision as of 21:16, 31 March 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: Dale Franco L. Caagbay - Protocol
Name: Dale Franco L. Caagbay - Protocol
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • Iphone 5
    • Flash: None
    • ISO setting: NA
    • White Balance: NA
    • Exposure: NA
    • Saturation: NA
    • Contrast: NA

Calibration

Photo of Fluorimeter


  • Distance between the smart phone cradle and drop = 8cm


Solutions Used for Calibration

Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (uL) Volume of the SYBR GREEN I Dye Solution (uL) Final DNA concentration in PicoGreen Assay (ng/mL)
0 80 80 blank
.25 80 80 .125
.5 80 80 .25
1 80 80 .5
2 80 80 1
5 80 80 2.5


Placing Samples onto the Fluorimeter

  • Get Slide, Micropipette and vial of sample
  • Place slide into fluorimeter
  • Take 80 micro liters of sample into micropipette
  • Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice (place new drops in different area)
  • Make sure the drops form a ball and don't spread around individually
  • Clean Up Workstation


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]




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