BME103 s2013:T900 Group6 L2: Difference between revisions
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''The overall procedure of the fluorescence technique consisted of an extremely hydrophobic (water-hating) surface. This was used in order to not allow any water to be absorbed during the experiment. The slide atop was coated with an adhesive cover in order to keep the molecules in place during analysis, and keep them a certain distance from the slide at the same time. This being so, it alloweed for the blue LED light to be focused in the drop; causing an increase in intensity which helped create the fluorescent environment. In addition, The SBYR Green dye that was added with the solvent helped form a complex with DNA that preferred to stick to the surface than anywhere else on the Fluorimeter. If a water-soluble dye was used instead, the outcome of the experiment would differ because instead of the green glow, a colorful beam of light would have shown through. | ''The overall procedure of the fluorescence technique consisted of an extremely hydrophobic (water-hating) surface. This was used in order to not allow any water to be absorbed during the experiment. The slide atop was coated with an adhesive cover in order to keep the molecules in place during analysis, and keep them a certain distance from the slide at the same time. This being so, it alloweed for the blue LED light to be focused in the drop; causing an increase in intensity which helped create the fluorescent environment. In addition, The SBYR Green dye that was added with the solvent helped form a complex with DNA that preferred to stick to the surface than anywhere else on the Fluorimeter. If a water-soluble dye was used instead, the outcome of the experiment would differ because instead of the green glow, a colorful beam of light would have shown through. | ||
*Note that the group used SYBR Green due to the fact it seemed to be the most eco-friendly." | *Note that the group used SYBR Green due to the fact it seemed to be the most eco-friendly." | ||
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Revision as of 23:37, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
Materials Smart Phone Camera Settings
Calibration Photo of Fluorimeter
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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